ABSTRACT
OBJECTIVE@#To study the changes of liver phosphofructokinase-2 (PFK-2) level at different postmortem intervals as well as due to different causes of death.@*METHODS@#One hundred and five rats were randomly divided into 3 groups and the rats were sacrificed by either bleeding, suffocating, and neck breaking, respectively. The content of liver PFK-2 at 0, 1, 2, 4, 8, 12 and 24 hours following death were studied using immunohistochemishtry and image analysis.@*RESULTS@#PFK-2 content of the rat's liver in all 3 groups showed a linear decrease at different postmortem intervals with no significant statistical differences found between the different groups.@*CONCLUSION@#PFK-2 level in rat liver appears to decrease linearly in correlation with prolonged PMI.
Subject(s)
Animals , Female , Male , Rats , Asphyxia/metabolism , Cause of Death , Cervical Vertebrae/injuries , Immunohistochemistry , Liver/enzymology , Phosphofructokinase-2/metabolism , Postmortem Changes , Random Allocation , Rats, Sprague-Dawley , Shock, Hemorrhagic/metabolism , Staining and Labeling , Time FactorsABSTRACT
OBJECTIVE@#To study the relationship between the expression of c-Fos protooncogene and skeleton muscle contusion of rats, and to search for a sensitive marker of timing for skeleton muscle contusion.@*METHODS@#Fifty Sprague-Dawley rats were randomly distributed into control group and experimental groups. The expression of c-fos was microscopically observed by immunohistochemical method.@*RESULTS@#With the time prolonged the c-fos positive intensity and area were increased. Positive expression of c-fos protein appeared at 15 min after skeleton muscle contusion, and reached to the peak at 1h after skeleton muscle contusion, then decreased gradually and returned to the normal level on 1d after skeleton muscle contusion.@*CONCLUSION@#The detection of the expression of c-fos protein could be a sensitive marker for timing skeleton muscle contusion.
Subject(s)
Animals , Female , Male , Rats , Contusions/pathology , Hindlimb/injuries , Immunohistochemistry , Muscle, Skeletal/pathology , Proto-Oncogene Proteins c-fos/biosynthesis , Random Allocation , Rats, Sprague-Dawley , Staining and Labeling , Time FactorsABSTRACT
OBJECTIVE@#To provide the evidence of the relationship between brain injury and the time of injury.@*METHODS@#Rats were contused on brain by fluid percussion, then were killed after injury for 15 min, 30 min, 1,3,6,12 h, and 1,4,7,14 d respectively. The expression of caspase-8 were detected by immunohistochemical technology on rat brain section and the results were assessed by image analysis system in the cerebral cortex, thalamus, and hippocampus.@*RESULTS@#The expression of caspase-8 in cortex and hippocampus could be detected in 30 min after injury, increased significantly in 3h, reached apex in 1d after injury, remained 4d before decreased. In addition, the expression of caspase-8 can be detected in 1h after injury and reached apex in 1d after injury, and remained 4d then reduced.@*CONCLUSION@#It seems that the expression of caspase-8 should be a useful target for diagnosis of early brain injury.